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Laboratory Tests in the Management of HIV Infection

Plasma Viral RNA Load

Ultrasensitive Viral Load Assays

Viral Load Below 500 Predicts Duration of Virologic Response

Roche HIV-1 Monitor

Chiron bDNA v.3

- novel chemistry gives higher specificity

 

Organon Teknika Nuclisens

   - Separates RNA from PCR inhibitors

   - Better quantitation in Tissues

   - Automated Extractor may improve throughput

Comparison Ultrasensitive Assays

Summary Ultrasensitive VL

The Value of Viral Culture

 

Definition of Viral Drug Resistance

 

Methods of Measuring Resistance

-Sequencing of PR and RT

 

Mutation Nomenclature

 

Genotypic Patterns of Resistance

Comparison of Methods for Viral Resistance Genotype

The Problem of the Minor Variant

 

Methods of Measuring Resistance

Comparison of Phenotyping Methods

Conclusions